Journal: Journal of Cellular and Molecular Medicine

Article Title: Mitochondrial implications in human pregnancies with intrauterine growth restriction and associated cardiac remodelling

doi: 10.1111/jcmm.14282

Figure Lengend Snippet: Sirtuin3 protein levels in placenta of the study groups. A, A representative Western Blot of Sirtuin3 protein expression in human placenta is shown in both controls (1‐3) and IUGR pregnancies (4‐6). β‐actin was used as the loading control. B, Graph showing the significant increase of Sirtuin3 levels (Sirt3/β‐actin ratio) in IUGR pregnancies (grey bars) compared with controls (empty bars). Results are expressed as mean ± SEM. Mann‐Whitney tests were used to seek for statistical analysis between groups. AU, Arbitrary units; IUGR, Intrauterine growth restriction; Sirt3, Sirtuin3; * P < 0.05

Article Snippet: The membranes were hybridised with anti‐Sirtuin3 (44 KD; 1:250; Merck Millipore, Burlington, MA, USA) overnight and at 4°C.

Techniques: Western Blot, Expressing, MANN-WHITNEY

Journal: Journal of Cellular and Molecular Medicine

Article Title: Mitochondrial implications in human pregnancies with intrauterine growth restriction and associated cardiac remodelling

doi: 10.1111/jcmm.14282

Figure Lengend Snippet: Association between clinical data and experimental results from pregnancies complicated by IUGR and controls. Decreased birth weight characteristic of IUGR is associated to increased BNP levels (as sign of cardiovascular remodelling) (A), decreased MRC CI in placenta (B) and enhanced Sirtuin3 protein expression (C). Additionally, impairment in MRC CI in placenta was directly associated to increased neonatal BNP levels (D) and placental Sirtuin3 protein expression (E), demonstrating the strong association among all these parameters. Spearman Rho tests were used to seek for statistical analysis. AU, Arbitrary units; BNP, Brain natriuretic peptide; CBMC, Cord blood mononuclear cells; CI, MRC complex I; g, grams; IUGR, intrauterine growth restriction; MRC, Mitochondrial respiratory chain; Sirt3, Sirtuin3

Article Snippet: The membranes were hybridised with anti‐Sirtuin3 (44 KD; 1:250; Merck Millipore, Burlington, MA, USA) overnight and at 4°C.

Techniques: Expressing

Journal: Journal of Cellular and Molecular Medicine

Article Title: Mitochondrial implications in human pregnancies with intrauterine growth restriction and associated cardiac remodelling

doi: 10.1111/jcmm.14282

Figure Lengend Snippet: Mitochondrial and cardiac features of human pregnancies in two contexts, complicated by intrauterine growth restriction (right side in red) and with no apparent obstetric problems (left side in green). In IUGR new‐borns, low birth weight was associated to a higher levels of BNP in new‐borns (indicating a potential cardiac remodelling), lower mitochondrial complex I function in placenta and decreased neonatal citrate synthase activity (representative of metabolic mitochondrial activity). In this context, there was an increase of placental protein Sirtuin3 levels, probably as a potential adaptation mechanism aimed to modulate the adverse mitochondrial phenotype reported in this obstetric complication. On the other hand, all these mitochondrial parameters were found within normal ranges in healthy children with no apparent IUGR. BNP, brain natriuretic peptide; CI, mitochondrial respiratory chain complex I; IUGR, intrauterine growth restriction

Article Snippet: The membranes were hybridised with anti‐Sirtuin3 (44 KD; 1:250; Merck Millipore, Burlington, MA, USA) overnight and at 4°C.

Techniques: Activity Assay

Journal: International Journal of Molecular Medicine

Article Title: Abnormal expression of SIRTs in psoriasis: Decreased expression of SIRT 1-5 and increased expression of SIRT 6 and 7

doi: 10.3892/ijmm.2019.4173

Figure Lengend Snippet: Primers used for human reverse transcription-quantitative polymerase chain reaction in the present study.

Article Snippet: Cultured HaCaT cells were incubated with the following specific antibodies overnight at 4°C: SIRT1 (1:50; cat. no. 13161-1-AP; ProteinTech Group, Inc., Chicago, IL, USA), SIRT2 (1:100; cat. no. PB0174; Wuhan Boster Biological Technology, Ltd., Wuhan, China), SIRT3 (1:100; cat. no. A01061-1; Wuhan Boster Biological Technology, Ltd.), SIRT4 (1:100; cat. no. ab10140; Abcam, Cambridge, MA, USA), SIRT5 (1:100; cat. no. 15122-1-AP; ProteinTech Group, Inc.), SIRT6 (1:100; cat. no. PB0375; Wuhan Boster Biological Technology, Ltd.) and SIRT7 (1:100 cat. no. PB0376; Wuhan Boster Biological Technology, Ltd.).

Techniques: Polymerase Chain Reaction, Sequencing

Journal: International Journal of Molecular Medicine

Article Title: Abnormal expression of SIRTs in psoriasis: Decreased expression of SIRT 1-5 and increased expression of SIRT 6 and 7

doi: 10.3892/ijmm.2019.4173

Figure Lengend Snippet: Primers used for mouse reverse transcription-quantitative polymerase chain reaction in the present study.

Article Snippet: Cultured HaCaT cells were incubated with the following specific antibodies overnight at 4°C: SIRT1 (1:50; cat. no. 13161-1-AP; ProteinTech Group, Inc., Chicago, IL, USA), SIRT2 (1:100; cat. no. PB0174; Wuhan Boster Biological Technology, Ltd., Wuhan, China), SIRT3 (1:100; cat. no. A01061-1; Wuhan Boster Biological Technology, Ltd.), SIRT4 (1:100; cat. no. ab10140; Abcam, Cambridge, MA, USA), SIRT5 (1:100; cat. no. 15122-1-AP; ProteinTech Group, Inc.), SIRT6 (1:100; cat. no. PB0375; Wuhan Boster Biological Technology, Ltd.) and SIRT7 (1:100 cat. no. PB0376; Wuhan Boster Biological Technology, Ltd.).

Techniques: Polymerase Chain Reaction, Sequencing

Journal: International Journal of Molecular Medicine

Article Title: Abnormal expression of SIRTs in psoriasis: Decreased expression of SIRT 1-5 and increased expression of SIRT 6 and 7

doi: 10.3892/ijmm.2019.4173

Figure Lengend Snippet: mRNA expression levels of SIRTs determined by reverse transcription-quantitative polymerase chain reaction in (A) cells, (B) mouse tissue and (C) human tissue. The expression levels of SIRT1, SIRT2, SIRT3, SIRT4 and SIRT5 in the experimental groups/patients with psoriasis were significantly reduced and those of SIRT6 and SIRT7 were significantly increased compared with the control groups. Error bars present the standard deviation. * P<0.05, ** P<0.01 and *** P<0.001 vs. the control group. SIRT, Sirtuin.

Article Snippet: Cultured HaCaT cells were incubated with the following specific antibodies overnight at 4°C: SIRT1 (1:50; cat. no. 13161-1-AP; ProteinTech Group, Inc., Chicago, IL, USA), SIRT2 (1:100; cat. no. PB0174; Wuhan Boster Biological Technology, Ltd., Wuhan, China), SIRT3 (1:100; cat. no. A01061-1; Wuhan Boster Biological Technology, Ltd.), SIRT4 (1:100; cat. no. ab10140; Abcam, Cambridge, MA, USA), SIRT5 (1:100; cat. no. 15122-1-AP; ProteinTech Group, Inc.), SIRT6 (1:100; cat. no. PB0375; Wuhan Boster Biological Technology, Ltd.) and SIRT7 (1:100 cat. no. PB0376; Wuhan Boster Biological Technology, Ltd.).

Techniques: Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Control, Standard Deviation

Journal: International Journal of Molecular Medicine

Article Title: Abnormal expression of SIRTs in psoriasis: Decreased expression of SIRT 1-5 and increased expression of SIRT 6 and 7

doi: 10.3892/ijmm.2019.4173

Figure Lengend Snippet: Protein expression levels of SIRTs determined by western blotting in (A) cells, (B) mouse tissue and (C) human tissue. The protein expression levels of SIRT1, SIRT2, SIRT3, SIRT4 and SIRT5 in the experimental groups/patients with psoriasis were significantly reduced and those of SIRT6 and SIRT7 were significantly increased compared with the controls. Error bars present the standard deviation. * P<0.05, ** P<0.01 and *** P<0.001 vs. the control group. SIRT, Sirtuin.

Article Snippet: Cultured HaCaT cells were incubated with the following specific antibodies overnight at 4°C: SIRT1 (1:50; cat. no. 13161-1-AP; ProteinTech Group, Inc., Chicago, IL, USA), SIRT2 (1:100; cat. no. PB0174; Wuhan Boster Biological Technology, Ltd., Wuhan, China), SIRT3 (1:100; cat. no. A01061-1; Wuhan Boster Biological Technology, Ltd.), SIRT4 (1:100; cat. no. ab10140; Abcam, Cambridge, MA, USA), SIRT5 (1:100; cat. no. 15122-1-AP; ProteinTech Group, Inc.), SIRT6 (1:100; cat. no. PB0375; Wuhan Boster Biological Technology, Ltd.) and SIRT7 (1:100 cat. no. PB0376; Wuhan Boster Biological Technology, Ltd.).

Techniques: Expressing, Western Blot, Standard Deviation, Control

Journal: International Journal of Molecular Medicine

Article Title: Abnormal expression of SIRTs in psoriasis: Decreased expression of SIRT 1-5 and increased expression of SIRT 6 and 7

doi: 10.3892/ijmm.2019.4173

Figure Lengend Snippet: IF in human skin lesions. IF in human psoriasis skin lesions was negative for (A) SIRT1, (B) SIRT2, (C) SIRT3, (D) SIRT4 and (E) SIRT5 antibodies. Lesions were positive for (F) SIRT6 and (G) SIRT7 antibodies. SIRTs were mainly observed in the epithelial layer. IF, immunofluorescence; SIRT, Sirtuin; FITC, fluorescein isothiocyanate.

Article Snippet: Cultured HaCaT cells were incubated with the following specific antibodies overnight at 4°C: SIRT1 (1:50; cat. no. 13161-1-AP; ProteinTech Group, Inc., Chicago, IL, USA), SIRT2 (1:100; cat. no. PB0174; Wuhan Boster Biological Technology, Ltd., Wuhan, China), SIRT3 (1:100; cat. no. A01061-1; Wuhan Boster Biological Technology, Ltd.), SIRT4 (1:100; cat. no. ab10140; Abcam, Cambridge, MA, USA), SIRT5 (1:100; cat. no. 15122-1-AP; ProteinTech Group, Inc.), SIRT6 (1:100; cat. no. PB0375; Wuhan Boster Biological Technology, Ltd.) and SIRT7 (1:100 cat. no. PB0376; Wuhan Boster Biological Technology, Ltd.).

Techniques: Immunofluorescence

Journal: International Journal of Molecular Medicine

Article Title: Abnormal expression of SIRTs in psoriasis: Decreased expression of SIRT 1-5 and increased expression of SIRT 6 and 7

doi: 10.3892/ijmm.2019.4173

Figure Lengend Snippet: IF in mouse skin lesions. IF in mouse skin lesions were also negative for (A) SIRT1, (B) SIRT2, (C) SIRT3, (D) SIRT4 and (E) SIRT5 antibodies. Lesions were positive for (F) SIRT6 and (G) SIRT7 antibodies. SIRTs were mainly localized in the epithelial layer. IF, immunofluorescence; SIRT, Sirtuin; FITC, fluorescein isothiocyanate.

Article Snippet: Cultured HaCaT cells were incubated with the following specific antibodies overnight at 4°C: SIRT1 (1:50; cat. no. 13161-1-AP; ProteinTech Group, Inc., Chicago, IL, USA), SIRT2 (1:100; cat. no. PB0174; Wuhan Boster Biological Technology, Ltd., Wuhan, China), SIRT3 (1:100; cat. no. A01061-1; Wuhan Boster Biological Technology, Ltd.), SIRT4 (1:100; cat. no. ab10140; Abcam, Cambridge, MA, USA), SIRT5 (1:100; cat. no. 15122-1-AP; ProteinTech Group, Inc.), SIRT6 (1:100; cat. no. PB0375; Wuhan Boster Biological Technology, Ltd.) and SIRT7 (1:100 cat. no. PB0376; Wuhan Boster Biological Technology, Ltd.).

Techniques: Immunofluorescence

Journal: International Journal of Molecular Medicine

Article Title: Abnormal expression of SIRTs in psoriasis: Decreased expression of SIRT 1-5 and increased expression of SIRT 6 and 7

doi: 10.3892/ijmm.2019.4173

Figure Lengend Snippet: IF in TNF-α-stimulated HaCaT cells was negative except for SIRT6 and 7. (A) SIRT1 and (B) SIRT2 were located predominantly in the nucleus and cytoplasm. (C) SIRT3, (D) SIRT4 and (E) SIRT5 were primarily mitochondrial proteins. (F) SIRT6 and (G) SIRT7 were mainly nuclear Sirtuins. IF, immunofluorescence; SIRT, Sirtuin; FITC, fluorescein isothiocyanate.

Article Snippet: Cultured HaCaT cells were incubated with the following specific antibodies overnight at 4°C: SIRT1 (1:50; cat. no. 13161-1-AP; ProteinTech Group, Inc., Chicago, IL, USA), SIRT2 (1:100; cat. no. PB0174; Wuhan Boster Biological Technology, Ltd., Wuhan, China), SIRT3 (1:100; cat. no. A01061-1; Wuhan Boster Biological Technology, Ltd.), SIRT4 (1:100; cat. no. ab10140; Abcam, Cambridge, MA, USA), SIRT5 (1:100; cat. no. 15122-1-AP; ProteinTech Group, Inc.), SIRT6 (1:100; cat. no. PB0375; Wuhan Boster Biological Technology, Ltd.) and SIRT7 (1:100 cat. no. PB0376; Wuhan Boster Biological Technology, Ltd.).

Techniques: Immunofluorescence

Journal: Frontiers in Pharmacology

Article Title: Improved myocardial mitochondrial energy metabolism in rats with chronic heart failure by modifying fatty acid oxidation using an extract of sand-fired aconite (Jianchang gang processing)

doi: 10.3389/fphar.2025.1600410

Figure Lengend Snippet: Effects of captopril and different processed aconite products on the AMPK, PGC-1α, and SIRT3 pathway in the myocardial tissue of CHF rats. (A) AMPK Western blot images. (B) Relative AMPK protein expression in the myocardial tissue of rats in each group ( n = 3). (C) p-AMPK Western blot images. (D) Relative p-AMPK protein expression in the myocardial tissue of rats in each group ( n = 3). (E) PGC-1α Western blot images. (F) Relative PGC-1α protein expression in the myocardial tissue of rats in each group ( n = 3). (G) SIRT3 Western blot images. (H) Relative SIRT3 protein expression in the myocardial tissue of rats in each group ( n = 3). # P < 0.05 and ## P < 0.01 in comparisons with the control group; * P < 0.05, ** P < 0.01, and *** P < 0.001 in comparisons with the CHF model group.

Article Snippet: Sirtuin 3 (SIRT3) antibodies (Batch No. 10099-1-AP), peroxisome proliferator-activated receptor coactivator-1α (PGC-1α) antibodies (Batch No. 66369-1-Ig), and carnitine palmitoyl transferase 1 (CPT1) antibodies (Batch No. 15184-1-AP) were purchased from Wuhan Sanying Biotechnology Co., Ltd. Atrial natriuretic peptide (ANP) Kits (Batch No. MK6949A), brain natriuretic peptide (BNP; Batch No. MK1608A), norepinephrine (NE; Batch No. MK2097A), malondialdehyde (MDA; Batch No. MK6877A), superoxide dismutase (SOD; Batch No. MK1927A), sodium-potassium-ATPase (Na + -k + -ATPase; Batch No. MK6748A), calcium-magnesium-ATPase (Ca 2+ -Mg 2+ -ATPase; Batch No. MK0007RA), adenosine triphosphate (ATP; Batch No. MK6913A), and free fatty acid (FFA; Batch No. MK1865A) kits were purchased from Jiangsu Sumeike Biotechnology Co., Ltd. We used a Fresco17 refrigerated centrifuge (Thermo Fisher company), Vevo2100 ultra-high resolution small animal color doppler ultrasound real-time imaging system (Visual Sonics company), Paraffin embedding machine, EG1150 (Leica company), Paraffin slicer RM2016 (Leica company), MF43 Microscope (Mingmei photoelectric technology Co., Ltd.), MC50 digital imaging measurement and analysis system (Mingmei photoelectric technology Co., Ltd.), Infinite F50 automatic enzyme label analyzer (Tecan company), Ultra-low temperature refrigerator (Haier company), and JEM-1400 transmission electron microscope (JEOL company) for the study.

Techniques: Western Blot, Expressing, Control

Journal: Frontiers in Pharmacology

Article Title: Improved myocardial mitochondrial energy metabolism in rats with chronic heart failure by modifying fatty acid oxidation using an extract of sand-fired aconite (Jianchang gang processing)

doi: 10.3389/fphar.2025.1600410

Figure Lengend Snippet: SFAS improves DOX-induced CHF via the AMPK-PGC-1α-SIRT3 pathway.

Article Snippet: Sirtuin 3 (SIRT3) antibodies (Batch No. 10099-1-AP), peroxisome proliferator-activated receptor coactivator-1α (PGC-1α) antibodies (Batch No. 66369-1-Ig), and carnitine palmitoyl transferase 1 (CPT1) antibodies (Batch No. 15184-1-AP) were purchased from Wuhan Sanying Biotechnology Co., Ltd. Atrial natriuretic peptide (ANP) Kits (Batch No. MK6949A), brain natriuretic peptide (BNP; Batch No. MK1608A), norepinephrine (NE; Batch No. MK2097A), malondialdehyde (MDA; Batch No. MK6877A), superoxide dismutase (SOD; Batch No. MK1927A), sodium-potassium-ATPase (Na + -k + -ATPase; Batch No. MK6748A), calcium-magnesium-ATPase (Ca 2+ -Mg 2+ -ATPase; Batch No. MK0007RA), adenosine triphosphate (ATP; Batch No. MK6913A), and free fatty acid (FFA; Batch No. MK1865A) kits were purchased from Jiangsu Sumeike Biotechnology Co., Ltd. We used a Fresco17 refrigerated centrifuge (Thermo Fisher company), Vevo2100 ultra-high resolution small animal color doppler ultrasound real-time imaging system (Visual Sonics company), Paraffin embedding machine, EG1150 (Leica company), Paraffin slicer RM2016 (Leica company), MF43 Microscope (Mingmei photoelectric technology Co., Ltd.), MC50 digital imaging measurement and analysis system (Mingmei photoelectric technology Co., Ltd.), Infinite F50 automatic enzyme label analyzer (Tecan company), Ultra-low temperature refrigerator (Haier company), and JEM-1400 transmission electron microscope (JEOL company) for the study.

Techniques: